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Recombinant Rat Interleukin-10
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| 性狀 | Recombinant Rat Interleukin-10 是由多種哺乳動物細胞類型產(chǎn)生的免疫抑制細胞因子，包括巨噬細胞，單核細胞，B細胞，T細胞和角質(zhì)形成細胞。
Synonyms rRtIL-10; CSIF Species Rat Source E. coli Accession P29456 Gene ID 25325 Molecular Weight Approximately 18.7 kDa AA Sequence MSKGHSIKGD NNCTHFPVSQ THMLRELRAA FSQVKTFFQK KDQLDNIVLT DSLLQDFKGY LGCQALSEMI KFYLVEVMPQ AENHGPEIKE HLNSLGEKLK TLWIQLRRCH RFLPCENKSK AVEQVKNDFN KLQDKGVYKA MNEFDIFINC IEAYVTLKMK N Biological Activity The ED50 is <0.5 μg/mL as measured by C6 cells, corresponding to a specific activity of >2.0 × 103 units/mg. Appearance Lyophilized powder. Formulation Lyophilized after extensive dialysis against PBS. Endotoxin Level <0.2 EU/μg, determined by LAL method. Reconstitution Reconstitute the lyophilized recombinant Rat Interleukin-10 (rRtIL-10) to 100 μg/mL using ddH2O. Storage & Stability Lyophilized recombinant Rat Interleukin-10 (rRtIL-10) is stored at -20°C. After reconstitution, it is stable at 4°C for 2 weeks or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping Room temperature in continental US; may vary elsewhere. Background Interleukin-10 is an anti-inflammatory Th1 immunosuppressive cytokine, the active form of which is a non-covalent homodimer, and which exhibits species-specificity both with respect to structure and biological activity. The rat homologue of IL-10 shares 73% identity with human IL-10 at the amino-acid sequence level, and has, in addition to the two disulphide bonds present in human IL-10, a fifth, unpaired cysteine (cys-149). Preparation of rat IL-10 by bacterial expression followed by solubilisation and refolding in a glutathione redox system, results in a molecule in which cys-149 is almost entirely oxidised, existing either as disulphide dimer or as a mixed disulphide with glutathione, and which has less than 1% of the activity of the native (cys-149-SH) form of the molecule. Site directed mutagenesis of rat IL-10 to replace cys-149 with tyrosine produces a molecule which readily adopts the active conformation upon solubilisation and refolding, and which is recoverable in good yield from bacterial expression systems. Comparison of the biological activities of rat IL-10tyr149 and commercial rat IL-10 preparations confirms that the activity of native-sequence rat IL-10 is either reduced or absent. It is proposed therefore that the biosynthetic analogue ratIL-10tyr149 is a more useful molecule to investigate the biological actions of IL-10 in the rat[1]. |
| 貯存 |
- Recombinant Rat Granulocyte-Macrophage Colony Stimulating Factor (CHO-expressed)
- Recombinant Rat Interferon-gamma
- Recombinant Rat Interleukin-1 alpha (CHO-expressed)
- Recombinant Rat Interleukin-1 beta
- Recombinant Rat Interleukin-10
- Recombinant Rat Interleukin-5
- Recombinant Rat Interleukin-6 (HEK293-expressed)
- Recombinant Rat Interleukin-7, His (CHO-expressed)
- Recombinant Rat Oncostatin-M
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